Host cells used for recombinant expression contain hundreds to thousands of host cell proteins (HCP) that can contaminate biopharmaceuticals. Failure to sufficiently reduce impurities in the early stages of drug development may reduce the effectiveness of the drug or cause adverse reactions in the patient. Regulatory guidelines require the reduction of HCP to the lowest possible levels.
Use our generic kits for host cell protein analysis:
During process and process development, validation to obtain relevant and specific data to drive process improvement and decrease HCP contamination.
During batch release testing and quality control (subject to extensive validation and qualification by each laboratory).
The antibodies in this kit were raised against and affinity-purified from a cell lysate of CHO cells grown in a medium supplemented with serum.
ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used application for detecting and quantifying proteins and antigens from various samples. Target-specific ELISA kits are available from a variety of manufacturers and can help optimize your immunodetection experiments.
The three Cygnus CHO HCP kits recognize a vast majority of the same HCPs, but because there is a possibility that the reported quantitative value for a given sample type may differ between kits, we cannot predict which kit will be the most suitable. for your applications.
Formal rating must be done on each different product to determine which of the three kits is the best. For labs examining CHO HCP impurities for the first time, use our CHO HCP ELISA, 3G (F550). If this method is unsuitable, suggest evaluating CHO HCP kits CM015 and F015.