Monocytechemotacticprotein–1 inhibits chondrogenesis of synovial mesenchymal progenitor cells: an in vitro study.
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Osteoarthritis (OA) is often progressive, painful disease of multifactorial. OA often develops with clear irreversible loss of articular cartilage, exposing the underlying bone, resulting in pain and loss of mobility. cartilage loss is considered permanent because the fixed ineffective and the apparent lack of stem cells / progenitor in the network.
However, the adjacent synovial lining and synovial fluid is abundant with mesenchymal progenitor / stem cells (synovial mesenchymal progenitor cells [sMPCs]) were able to differentiate into cartilage both in vitro and in vivo. Previous research has shown that the MPCs can return to factors such as monocyte chemotactic protein 1 (MCP-1 / CCL2) stated after the injury.
While MCP-1 (and the appropriate receptor) appears to play a role in the recruitment of stem cells to the site of injury, in this study, we have shown that MCP-1 upregulated in OA synovial fluid and that exposure to MCP-1 activates sMPCs, while simultaneously inhibiting cell this of undergoing chondrogenesis-cells in vitro. Furthermore, exposure to physiological (OA knee joint synovial fluid) levels of MCP-1 triggers a change transcriptome of sMPCs and prolonged exposure to induce the expression of the chemokine MCP-1 in sMPCs, generating positive feedback from the sMPCs can not seem to escape.
Therefore, we propose a model in which MCP-1 (usually expressed after joint injury) sMPCs recruited to the injured area, but at the same SMPC trigger changes in transcriptional regulation, leading to blockages in chondrogenic program. These results may open new avenues of research observed a lack of endogenous repair after injury to the articular cartilage and / or arthritis.
Monocytechemotacticprotein–1 inhibits chondrogenesis of synovial mesenchymal progenitor cells: an in vitro study.
Correlation of interleukin-6 and monocytes chemotactic protein – one concentration with the formation of crescent and myeloperoxidase special anti-neutrophil titer cytoplasmic antibodies in SCG / Kj mice by treatment with anti-interleukin-6 receptor antibody or mizoribine.
Special myeloperoxidase anti-neutrophil cytoplasmic antibody (MPO-ANCA) associated with rapidly progressive glomerulonephritis (RPGN) and glomerular crescent formation. pathogenic factor in RPGN analyzed by SCG / Kj mice, which spontaneously develop RPGN MPO-ANCA associated. The serum concentration of soluble IL-6R were determined by ELISA and others 23 cytokines and chemokines with Bio-Plex analysis.
Part of frozen kidney tissue examined with a fluorescence microscope and CD3 (+) B220 (+) T cells in the spleen part determined by flow cytometry. The concentration of IL-6 and monocyte chemotactic protein-1 was significantly correlated with the percentage of crescent formation. antibody anti-IL-6R, which has been effective in patients with rheumatoid arthritis, given to the SCG / Kj mice to elucidate the role of IL-6 in the development RPGN. MPO-ANCA titer decreased after administration of anti-IL-6R antibodies, but no titer of mizoribine, which is effective in Kawasaki disease model mice.
These results suggest that IL-6-mediated signaling is involved in the production of MPO-ANCA.The levels of MCP-1 mRNA and protein increased significantly after treatment with different concentrations of P.endodontalis LPS (0-50 mg / L), indicating that P.endodontalis LPS induced osteoblasts to express MCP-1 in a dose-dependent manners.
Description: A competitive ELISA for quantitative measurement of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Duck Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor ELISA Kit
During the observation period (0-48 h), the effects of 20 mg / L P.endodontalis LPS on the induction of MCP-1 in MC3T3-E1 cells exhibited a time-dependent manner. MCP-1 mRNA expression decreased significantly after pre-treatment with 10 mol / L SB203580 and BAY11-7082 for 1 hour, and the inhibitory effects of SB203580 stronger than BAY11-7082.
