Antidicer RNAse activity of monocytechemotacticprotein-induced protein–1 is critical for inducing angiogenesis.
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inflammatory angiogenesis involves ZC3H12A encoding gene induction of monocyte chemoattractant protein-1 Novel (MCP-1) induced protein-1 (MCPIP1) having deubiquitinase and antidicer RNase activity. Whether and how the enzymatic activity MCPIP1 MCPIP1 mediate biological function is unknown.
The study comes with human umbilical vein endothelial cells showed that MCPIP-induced angiogenesis mediated by hypoxia inducible factor (HIF-1α), vascular endothelial growth factor (VEGF), and silent information regulator (SIRT-1) induction resulted in inhibition of angiogenesis inhibitor thrombospondin- 1. MCPIP1 expression inhibits the production of antiangiogenic microRNA (mir) -20b and -34a which suppresses translation of HIF-1α and SIRT-1, respectively.
The MCPIP D141N mutant RNase-dead not only did not induce angiogenesis but also failed to inhibit the production of miR-20b and -34a shows that the RNase activity antidicer of MCPIP1 involved in angiogenesis-mediated MCPIP. Mimetics of miR-20b and -34a MCPIP1-induced angiogenesis inhibiting confirm that MCPIP1 pressing the biogenesis of miR-20b and -34a. Furthermore, our results indicate that MCPIP expression induces nuclear translocation of HIF-1α.
We show that under hypoxia angiogenesis mediated through induction MCPIP1 and under normoxia, in vitro, MCPIP deubiquitinates ubiquitinated HIF-1α, and stabilized HIF-1α into the nucleus to promote transcription of target genes, cyclooxygenase-2 and VEGF, suggesting that the activity deubiquitinase of MCPIP also can promote angiogenesis. These results show for the first time that the RNase activity antidicer of MCPIP1 very important in mediating the biological function MCPIP, ie angiogenesis.
Antidicer RNAse activity of monocytechemotacticprotein-induced protein–1 is critical for inducing angiogenesis.
[Effect monocytes chemotactic protein -3 ICAM- one , VCAM- one , TF and TFPI expression and apoptosis in human umbilical vein endothelial cells].
OBJECTIVE To determine the effect of monocyte chemotactic protein-3 (MCP-3) on the expression of adhesion between the molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), tissue factor (TF, and tissue factor pathway inhibitor (TFPI ) and apoptosis in human umbilical vein endothelial cells (HUVECs).
METHOD Cultured HUVECs treated with MCP-3 at a predetermined optimum concentration 1 hour after treatment with or without MCP-3 antibody (20 ng / ml), PI3K inhibitors, or LY-294 002 (5 mmol / ml). The expression of ICAM-1, VCAM-1, TF and TFPI was analyzed using RT-PCR and Western blot after treatment. MCP-3 mRNA and protein expression was detected in HUVECs exposed to 50 ug / ml ox-LDL for 24 hours. The cell apoptosis and caspase-3 protein production in HUVECs treated with MCP-3 or MCP-3 plus CCR2 antagonist for 24 hours and 48 hours was evaluated by flow cytometry and Western blotting.
RESULTS At the optimal concentration of 0.3 ng / ml, MCP-3 treatment for 24 hours due to significantly increased ICAM-1, VCAM-1, and the expression of TF by lowering expression of TFPI in HUVECs (P <0.05), and the effects such as by the application significantly inhibited MCP-3 antibody, PI3K inhibitor, or LY-294 002 (P <0.05). Ox-LDL exposure significantly increased the expression of MCP-3 in HUVECs (P <0.05). HUVECs showed significantly increased the level of apoptosis after treatment with MCP-3 or MCP-3 plus CCR2 antagonist (P <0.05), and the rate of apoptosis increased significantly as a long-term treatment (P <0.05); caspase-3 protein expression in the cell showed the same pattern of changes following the treatment.
Description: A competitive ELISA for quantitative measurement of Rat Monocyte Chemotactic Protein 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Monocyte Chemotactic Protein 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Monocyte Chemotactic Protein 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte Chemotactic Protein 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte Chemotactic Protein 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte Chemotactic Protein 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Monocyte Chemotactic Protein 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Monocyte Chemotactic Protein 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Monocyte Chemotactic Protein 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
CONCLUSION ox-LDL can cause MCP-3 expression in HUVECs. MCP-3 and induces apoptosis of HUVECs significantly affect cell function partly through the PI3K pathway signaling.
