Monocytechemotacticprotein–1 plays a role in ovarian dysfunction related to high-fat diet-induced obesity
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Obesity, known to cause elevation of systemic monocyte chemotactic protein-1 (MCP-1), adversely affecting normal ovarian function. The purpose of this study was to determine whether MCP-1 plays a role in ovarian dysfunction associated with obesity caused by a high fat (HF) diet intake. The wild-type (WT) C57BL / 6J mice were fed either a normal chow (NC) (Group 1, control group) or HF diet (Group 2).
To assess whether MCP-1 is involved in ovarian dysfunction HF-diet-induced, MCP-1 knockout mice fed the HF diet (Group 3). weight, body fat composition, the number of oocytes collected following ovarian superovulation with gonadotropins, ovarian macrophage markers and expression of genes important in folliculogenesis and steroidogenesis were measured in three groups of animals.
Animals in Group 2 gained significant weight and body mass, resulting in the fewest number of oocytes following superovulation, and has a significant change in the genes involved in ovarian folliculogenesis and steroidogenesis as well as genes involved in inflammation.
While the animals in Group 3 has a weight and body fat composition is highest, they produce the same number of oocytes compared to animals in Group 1, but has a different ovarian gene expression compared to Group 2. These findings indicate that MCP-1 knockout gene may reverse some of the adverse effects of obesity caused by the intake of the HF diet.
Future studies assessing ovarian histology in MCP-1 immobilize a mouse model will confirm our findings. MCP-1 inhibition may represent a future therapeutic target to protect the health of the ovaries from the adverse effects of HF diet consumption.
Monocytechemotacticprotein–1 plays a role in ovarian dysfunction related to high-fat diet-induced obesity
monocytes chemotactic protein – one induced protein one contribute to neuronal injury following hypoxia-ischemia in neonatal rat brain
Neuroinflammation has been involved in neurodegenerative diseases and acute brain injury such as stroke. Monocyte chemotactic protein-1-induced protein-1 (Mcpip1) is a multifunctional protein known to act pro-apoptotic or anti-apoptotic depending on the nature of the experimental arrangement. However, its role in brain damage after asphyxia in the developing brain has not been studied.
Therefore, we explored the role Mcpip1 on brain injury after hypoxia-ischemia in neonatal rats. At postnatal day 7, Mcpip1-deficient and wild-type mice carotid artery ligation and exposure to hypoxia (8% oxygen). After a hypoxic-ischemic insult, we determine the time-course of cell death of apoptosis and expression levels of genes that encode proinflammatory factors. Mcpip1 impact on long-term brain damage rated one week after hypoxia-ischemia by cresyl violet staining.
We found the caspase-3 activity increased significantly in the ipsilateral brain tissue within 12-24 hours after hypoxia-ischemia. There is a marked increase in levels of mRNA transcripts that encode Mcpip1, TNF, and CCL2 in the ipsilateral brain tissue 6-48 hours after hypoxia-ischemia. We found the hypoxia-ischemia-induced caspase-3 activity and levels of proinflammatory genes are inactivated in Mcpip1-KO mice compared to wild-type mice.
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Description: A competitive ELISA for quantitative measurement of Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Description: A competitive ELISA for quantitative measurement of Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Description: A competitive ELISA for quantitative measurement of Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Histological assessment revealed that hypoxia-ischemia-induced brain tissue loss was significantly attenuated in the hippocampus of Mcpip1-KO mice compared to wild-type mice (9.0 ± 5.6% vs. 33.9 ± 11.0%, P <0.05 ). Our data show that Mcpip1 contributes to acute and delayed brain damage, in part, through the regulation of neuroinflammation after hypoxic-ischemic insult in the developing rat brain.
